Moreover, the value of pH decreased with enrichment of VFAs, especially butanoic acid, because VFAs were dominant in the intermediates of co-degradation. The structures of bacteria and fungi also changed significantly in the process of co-degradation which mainly enriched macromolecules degrading and volatile fatty acids (VFAs) metabolizing microbes to facilitate methane generation. A shift of dominant methanogen in inoculum was observed in the process of co-degradation from acetoclastic Methanosaeta to methylotrophic Methanomethylovorans on the seventh day, finally to hydrogenotrophic Methanobacterium. The experimental results indicated that methane production in the cultivation CRS was about 12 times higher than that of C. In the laboratory tests, the succession of microbial communities and organic compounds in the process of methane generation from among degradations of coal (C), rice straw (RS), and coal-rice straw (CRS) were investigated by using with MiSeq and GC-MS analytical instruments. Compared with the biodegradation of coal, the co-degradation of coal and straw can significantly improve methane production, which is potential to increase biogenic coalbed methane (CBM) production.
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